Thematic plan of the microbiology, virology and immunology practical lessons icon

Thematic plan of the microbiology, virology and immunology practical lessons




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Thematic plan of the microbiology, virology and immunology practical lessons

for the students of stomatological faculty (speciality-stomatology)


N

les

Number of hours

Theme of practical classes

Credit N1. Morphology, physiology of microorganisms. Infection. Immunity.

Thematic credit 1. Introduction in microbiology.

1

2

Working regime of microbiological laboratory. The rules of the work in the bacteriological laboratory. Microscopy techniques. Immersion microscopy of bacterial smears.

^ Thematic credit 2. Morphology and structure of the prokaryotes and unicellular parasitic eucaryotes.

2

2

Morphology of bacteria. Preparation of the smear from bacterial culture. The simple method of staining.

3

2

Structure and chemical composition of bacterial cell. The structure of the cell wall. L-forms bacteria. The complete methods of staining. Gram’s method.

4

2

Acid-fast bacteria. Bacterial endospores. Methods of their staining with Aujesko`s and Zielh-Neelsen`s techniques.

5

2

Capsule of bacteria. Flagella. Intracellular inclusions. Methods of their revealing.

6

2

Morphology of spirochetes, rickettsia, chlamydia, mycoplasma and actinomycetes., features of their structure and methods of staining

^ Thematic credit 3. Physiology of the microorganisms. Evolution and classification of the germs.

7

2

Physiology of the microorganisms. Nutrition of bacteria. Isolation of a bacterial pure culture (first stage).

8

2

Physiology of the microorganisms. Multiplication of bacteria. Isolation of a bacterial pure culture (second stage).

9

2

Physiology of the microorganisms. Bacterial enzymes. Biochemical activity of bacteria. Isolation of a bacterial pure culture (third stage).

10

2

Physiology of the microorganisms. Bacterial respiration. Cultivation of anaeaerobs. Pure culture isolation of an anaerobic micrororganism.

^ Thematic credit 4. Bacterial genetics.

11

2

Bacterial genetics. Variation of bacteria. Brief review of the genetic engineering. Transformation. Transduction. Conjugation.

^ Thematic credit 5. Microbiological base of an antimicrobial chemotherapy and asepsis.

12

2

Influence of the physical and chemical factors on microorganisms. Sterilization techniques..

13

2

Chemotherapeutical preparations. Antibiotics. Determination of a bacterial susceptibility to antimicrobial drugs.

^ Thematic credit 6. Infection.

14

2

Infection. Factors of a bacterial pathogenicity, their detection. Biological method of a laboratory diagnostics.

Thematic credit 7. Immune system. Non-specific defense from pathogens.

15

2

Species immunity. Detection of the nonspecific resistance factors.

^ Thematic credit 8. Immunity reactions. Antigens and antibodies.Immunopathology.

16

2

Acquired (specific) immunity. Antigens and antibodies. Serological method of the laboratory diagnostics of infectious diseases. Agglutination test, its modifications. Precipitation tests, its modifications.

17

2

Serological tests. Complement fixation test. Serological tests with labelled antibody or antigen, their application in the laboratory diagnostics.

^ Thematic credit 9. Immunopathology. Immunoprophylaxis of infectious diseases.

18

2

Immunoprophylaxis and immunotherapy of infectious diseases. Vaccines and toxoides.




19

2

Immunoprophylaxis and immunotherapy of infectious diseases. Immune serums and immunoglobulin.

20

2

Summary control of the credit N1. Morphology, physiology of microorganisms. Infection. Immunity.

Credit N2. Special, clinical and ecological microbiology.

Thematic credit 10. Pathogenic prokaryotes and eucaryotes

21

2

Rickettsia. Biological properties. Laboratory diagnostics of rickettsiosis. Serological diagnostics of the epidemic and endemic (murine) typhus, Q-fever.

22

2

Pathogenic cocci. Staphylococci and streptococci. Morphology and biological properties. Laboratory diagnostics of suppurative and non-suppurative lesions caused by them.

23

2

Pathogenic Gram-negative cocci. Gonococci and meningococci. Biological characteristics and laboratory diagnostics of diseases.

24

2

Enterobacteria. Biological characteristics and classification of family. Characteristics of genera Escherichia, Shigella. Pathogenesis, laboratory diagnostics of coli-enteritis and bacterial dysentery.

25

2

Enterobacteria. Salmonella. Biological characteristics of Salmonella, causing typhoid and paratyphoid fevers. Pathogenesis and laboratory diagnostics of enteric and paratyphoid fevers. Pathogenesis and laboratory diagnostics of acute gastroenteritis caused by Salmonella.

26

2

Opportunistic enterobacteria: Klebsiella spp. and Proteus spp.Pseudomonas aeruginosa. Biological features. . Pathogenesis and laboratory diagnostics of opportunistic infections

27

2

Pathogenic Vibrio. Biological properties of Vibrio cholerae. Laboratory diagnostics of cholera and other infections caused by pathogenic vibrio.

28

2

Microorganisms causing zoonotic infections. Biological characteristics of Yersinia spp. Morphology and biological characteristics of Francisella tularensis. Bacillus anthracis. Pathogenesis and laboratory diagnostics of a plaque, enteric yersiniosis, pseudotuberculosis and tularemia and anthrax.

29

2

Medical-important clostridia. Biological characteristics Clostridium tetani and Clostridium botulinum. Pathogenesis, laboratory diagnostics and specific prevention of tetanus and botulism.

30

2

Anaerobic rods causing purulent wound infections and gas gangrene. Pathogenesis, laboratory diagnostics and specific prevention of gas gangrene and wound infections.

31

2

Corynebacterium diphtheria. Bordetella spp. Biological characteristics. Pathogenesis, laboratory diagnostics, specific therapy and prevention of diphtheria and whooping cough.

32

2

Mycobacteria. Causative agents of tuberculosis and leprosy, their biological properties. Pathogenesis, laboratory diagnostics, specific prevention of diseases.

33

2

Medical-important spirochetes. Biological characteristics of Treponema pallidum. Pathogenesis and laboratory diagnostics of syphilis.

34

2

Pathogenic actinomycetes and fungi of genus Candida. Morphology and biological properties. Laboratory diagnostics of infections caused by them.

35

2

The most medical-important protozoa. Causative agents of toxoplasmosis, amebiasis, thrichomoniasis. Features of laboratory diagnostics of protozoan infections.

^ Thematic credit 11. Clinical and ecological microbiology. Introduction.

36

2

Major tasks of the clinical microbiology. Normal microbiota of the human body. Pathologically changed microbiota, clinical appearance and laboratory revealing

^ Thematic credit 12. Sanitary microbiology and virology

37

2

Sanitary microbiology and virology, main principles. Sanitary-microbiological investigation of the water, soil and air.

38

2

Summary control of the credit N2. Special, clinical and ecological microbiology

Credit N3. General and special virology

Thematic credit 13. Main postulates of a virology

39

2

Virology. Morphology and structure of viruses. Methods of the virus cultivation. Indication and identification of viruses. Laboratory diagnostics of the viral infections.

^ Thematic credit 14. Special virology.

40

2

Viruses of the flu, measles and parainfluenza. Biological properties. Laboratory diagnostics of diseases caused by them.

41

2

Enteroviruses. Poliovirus, Coxsackie and ECHO viruses. Laboratory diagnostics of diseases.

42

2

Hepatitis viruses. Biological properties. Laboratory diagnostics of a human viral hepatitis.

43

2

Retroviruses. Human immunodeficiency virus. Laboratory diagnostics of the HIV infection and AIDS.

44

2

Herpes viruses. Biological properties. Laboratory diagnostics of the diseases caused by herpes viruses.

45

2

Summary control of the credit N3. General and special virology.



A chief of microbiology, virology and immunology department

Academician AS HS of Ukraine

Doctor of medicine, professor G.K.Paliy

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