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MINISTRY OF PUBLIC HEATH OF UKRAINE


BUKOVINIAN STATE MEDICAL UNIVERSITY

STUDENT’S GUIDE

for study Microbiology, Immunology and Virology


Student’s guide for study of Microbiology, Immunology and Viriology is provided by “Programme on Microbiology, Immunology and Viriology ”

for higher medical education establishments of Ukraine witg III-IV accreditation level (Ministry of Health of Ukraine, 2007)


“Microbiology, immunology and virology” is studied during for higher medical education establishments of Ukraine with III-IV accreditation level for specialities “Medicine” 7.110101, “Pediatry” 7.110104, “Prophylactic medicine” 7.110105, approved by Ministry of Public Health of Ukraine (order of MPH of Ukraine № 239 of 16.04.03), new experimental academic curriculum, developed under the concept of Europen Credit Transfer System (ECTS) approved by Ministry of Public Health of Ukraine (order № 52 of 31.01.2005).

Student workload on microbiology in ECTS consists of learning activities such

a) lectures; b) practical classes; c) individual self-work.

Topics of the lectures are devoted to the problem questions of appropriate chapters of infection diseases, ecology of environment, immunology.

  1. Introduction

Amount of hours –240 (8 credits ECTS) include:

Lectures – 50 hours

Practical lessons – 120 hours

Individual student’s self-work – 70 hours

Structure of subject consist of 3 modules and 14 contents modules

Studying plan of the subject “^ Microbiology, virology, immunology

for 2-3 years student of the medical faculty

Structure of subject

Amount of ours, from that

Years of studying

Type of the control

Total

In classes

ISW


lectures

Practical classes




240

50

120

70

2




Credits ЕСТS

8















Module 1


(Contents modules 9):

90 ours/

3,0 credits ЕСТS

14

40

36

2

Final

module control

Module 2


(Contents modules 3):

90 ours /

3,0 credits ЕСТS

26

50

14

2

Module 3


(Contents modules 2)


60 /

2,0 credits ЕСТS

10

30

20

3

1 credit ЕСТS - 30 ours, ISW – individual self-work

  1. ^ Structure of subject “Microbiology Immunology and Virology”




Module 1 Morphology and physiology of microorganisms. Infection. Immunity


Module 2

Special, clinic and ecological microbiology

Module 3

General and special virology

Amount of the contents modules

9 (1-9)

3 (10-12)

2 (13-14)

Amount of practical lessons

19

23

14

Traditional marks
^
Marks in scores

“5”

6

5

8

“4”

5

4

6

“3”

4

3

5

“2”

0

0

0
^
Individual work

6

5

8

Minimal scores

76

74

70

Module 1. Morphology and physiology of microorganisms. Infection. Immunity

Contents modules:

1. Introduction in microbiology.

2. Morphology and structure of prokaryotes and monocellular parasite eukaryotes.

3.Physiology of bacterium. Evolution and classification of microorganisms.

4.Genetics of microorganisms.

5. Microbiologycal basis of antimicrobial chemotherapy.

6. Infection.

7. Human immune system. Nonspecific immune response.

8. Antigens. Antibody.

9. Disoders of the immune system. Diagnostic testing techniques.

Мodule 2. Special, clinical and ecological microbiology

Contents modules:

10. Pathogenic prokaryotes and eukaryotes.

11. Basis of clinical and ecological microbiology.

12. Sanitary microbiology and virology.

Module 3. General and special virology

Contents modules:

13. General virology.

14. Special virology.

Plan of the lectures


Topic

Ours

Module 1. Morphology and physiology of microorganisms. Infection. Immunity.



Historical Outline of the Development of Microbiology. Classification of microorganisms. Morphology of bacteria.

2



Antimicrobial medicines. Antibiotics. Bacteriophages.

2



Infection and Infectious Process.

2



Historical aspects of the development of immunology. Nonspecific immune response.

2



Human immune system. Antigens, properties. Antigens of microorganisms.

2



Antibody, structure. Classes of immunoglobulins. Ruls of immune response. Сooperation of the cells in the specific immune response.

2



Disoders of the immune system. Immune prophylaxis and immunotherapy .

2

Module 2. Special, clinical and ecologycal microbiology

8.

Special medical microbiology. Modern methods of laboratory diagnostic of the infectious diseases.

2



Gram-positive and Gram-negative pyogenic cocci.

2



Conditionaly pathogenic enterobacteria: escherichia, proteus, klebsiella,yersinia.

2



Pathogenic enterobacteria: salmonella (typhus and paratyphus, gastroenteritis).

2



Causative agents of bacterial dysentery. Causative agents of cholera.

2



Causative agents of plaque and anthrax.

2



Causative agent of “air-dropled infectious”: corynebacterium diphtheria.

2



Causative agent of “air-dropled infectious”: mycobacterium tuberculosis.

2



Pathogenic protozoa. Laboratory diagnosis of protozoal diseases.

2



Riccetsia. Chlamidia. Mycoplasma.

2



Ecological and sanitary microbiology.

2



Clinical microbiology. Nosocomial infections.

2




Module 3.General and special virology





^

Morphology and ultrustructure of viruses. Cultivation of viruses.


2


Family of Picornaviridae. Enteroviruses: virusses of epidemic poliomyelitis, Coxakie, ECHO.


2


^

Families of Orthomyxoviruses and Paramyxoviruses.


2


Families of Herpesviridae, Adenoviridae.


2


^

Family of Retroviruses. AIDS. Oncogenic viruses.


2




Total ours

50

plan of the practical classes



Theme

Ours

Module 1. Morphology and physiology of microorganisms. Infection. Immunity.

1.

Arrangement, equipment and safety rules in the microbiology laboratory. Microscope examination of prepared slide.

2

2-3.

Morphology and physiology of bacteria. Microscopic technique: staining (simple and special methods).

4

4.

Aseptic surgery, antiseptic methods and stuffs. Sterilization and disinfection.

2

5.

Physiology of bacteria. Metabolism of bacteria. Nutrient media.

2

6.

Physiology of bacteria. Separation and identification of aerobic microorganisms (1-st day of the student’s research work).

2

7.

Cultural properties of bacteria. Separation and identification of aerobic microorganisms (2-nd day of the student’s research work).

2

8.

Biochemical properties of bacteria. Separation and identification of aerobic microorganisms (3-d day of the student’s research work).

2

9.

Particularities of separation and identification of anaerobic micro-organisms. Separation and identification of aerobic micro-organisms (4th day of the student’s research work).

2

10.

Genetics of micro-organisms.

2

11.

Microbiologic basis of antimicrobial chemotherapy. Antibiotics.

2

12.

Viruses of bacteria. Bacteriophages. The methods of detection and titration of phages.

2

13.

Infection and Infectious Process.

2

14.

Organs of immune system. Nonspecific response versus.

2

15.

Antigens and antibodies.

2

16-17.

Diagnostic testing techniques. Serologycal tests.

4

18.

Diagnostic testing techniques with marks.

2

19.

Vaccines and immune serums.

2

20.

Final module control.

2

Module 2. Special, clinical and ecologycal microbiology

1.

Laboratory diagnostic of Staphilococcal diseases. 1st day of student’s scientific research (SSR).

2

2.

Laboratory diagnostic of Streptococcal diseases. 2nd day of student’s scientific research (SSR).

2

3.

Laboratory diagnostics of diseases, caused by pathogenic Neisseria. 3d day of student’s scientific research (SSR).

2

4.

Laboratory diagnostics of diseases, caused by Clostridium. Tetanus. Botulism. Conclusion of SSR.

2

5.

Laboratory diagnostics of diseases, caused by pathogenic protozoa.

2

6.

Laboratory diagnostics of diseases, caused by fungi.

2

7.

Escherichioses. Laboratory diagnostic.

2

8.

Laboratory diagnostic of typhoid fever, paratyphoids and food toxinfections, caused by Salmonellae.

2

9.

Laboratory diagnostic of bacterial dysentery (shigellosis).

2

10.

Laboratory diagnostics of diseases causes by conditional pathogenic microorganisms (Klebsiella, Proteus, Pseudomonas aeruginosa)

2

11.

Laboratory diagnostic of cholerae.

2

12.

Laboratory diagnostic of yersineosis.

2

13.

Laboratory diagnostic of brucellosis and tularemia.

2

14.

Laboratory diagnostic of Anthrax.

2

15.

Laboratory diagnostic of diphtheria.

2

16.

Laboratory diagnostic of tuberculosis and leprosy.

2

17.
^

Laboratory diagnostic of syphilis, borrelioses and leptospiroses.


2

18.

Laboratory diagnostic of relapsing fever, Brill-Cinsser's disease, Q-fever.

2

19.

Laboratory diagnostic of chlamidioses and mycoplasmoses.

2

20.

Microbiology and ecology of environments.Microflora of water, soil, air. Methods of detection of the bacterial pollution.

2

21.

Microflora of the human body. Dysbacteriosis. Gnotobiology.

2

22.

Clinical microbiology . Nosocomial infections.

2

23.

Microbiological diagnosis of nosocomial infections.

2

24.
^

Final module control.


4




Module 3.General and special virology




1.

Morphology and cultivation of viruses.

2

2.

Laboratory diagnostic of epidemic poliomyelitis. Student’s research work (1st day).

2

3.

Laboratory diagnostic of diseases caused by Coxackie and ECHO viruses. Student’s research work (2nd day).

2

4.

Laboratory diagnostic of diseases caused by rhinovirises, aphthoviruses and reoviruses. Student’s research work (3rd day).

2

5.

Laboratory diagnostic of diseases caused by Arboviruses and Rhabdoviridae.

2

6.

Influenza viruses. Laboratory diagnostic.

2

7.

Parainfluenza viruses. Virus of epidemic parotitis. Laboratory diagnostic.

2

8.

Morbilivirus (Measles), Pneumoviruses (Respiratory Syncitium viruses). Laboratory diagnostic.

2

9.

Smallpoxviruses.

2

10-11.
^

Herpesviridae. Adenoviruses. Laboratory diagnostic.


4

12-13.

Viruses – causative agents of hepatitis.


4

14.

Oncogenic viruses. Family retroviruses. AIDS.

2

15.

Final module control 3

2




Total amount of ours (practical classes)

120




including final module control ( of 3 modules)

8

^ Plan of self student work



Тheme

ours

Type of control

Module 1. Morphology and physiology of microorganisms. Infection. Immunity.

1.

Preparation to the practical classes (theoretical and practical trening)

21

Control on practical lessons

2.

Self working with the topics, which were not put into the plan of practical classes







Steps of development of microbiology

1

Final module control

Immunopathology. Analise of the immune state of human body

2

Final module control

3.

Individual self-work: to make the scheme of cell’s cooperation in immune response

2

Control on practical lessons

4.

Preparing to the final module control 1

10

Final module control

Module 2. Special, clinical and ecologycal microbiology

1.

Preparation to the practical classes (theoretical and practical trening).

4

Control on practical lessons

2.

Self working the topic, which was not putting into the plan of practical classes:







Anaerobic nonclostridial bacteria.

1

Final module control

Causative agent of whooping-cough

1

Final module control

Gram-negative, non-fermentation bacteria

1

Final module control

Other pathogenic bacteria

2

Final module control

3.

Preparing to the final module control 2

5

Final module control

Module 3. General and special virology

1.

Preparing to the practical classes (theoretical and practical traning)

11

Control on the practical classes

2.

Self work under the topic which were not put to the plane of practical lessons:










Other RNA-consist viruses

2

Final module control




Ecologycal group of arbovirueses.

2

Final module control

3

Preparing to the final module control 3

5

Final module control




Total

20







Amount of ours of SSW from subject

70







Including preparing to the final modules controls(3)

20




Assesment criteria

^ TRADITIONAL MARK

Convertation into scores

Module 1

Module 2

Module 3

“5”

6

5

8

“4”

5

4

6

“3”

4

3

5

“2”

0

0

0

Individual self-work

6

5

8

Maximal scores for practical classes

120

120

120

Maximal scores for final control

80

80

80

Maximal total scores for module

200

200

200

Final module control

perfomes on the last practical classs of each module. It consists of 2 parts: theoratical and practical. Maximal scores which student may have for theoretical questions - 50, for practical – 30.




The hiest scores

Theoretical part

Practical part

Module 1

80

5 questions:

“exellent” 10 scores

“good” 8 scores

“satisfactory ” 7 scores

3questions

“exellent” 10 scores

“good” 8 scores

“satisfactory” 7 scores

Module 2

80

20 tests “Krok 1”20 scores

4 questions:

“exellent” 9 scores

“good” 7 scores

“satisfactory ” 6 scores


3 questions

“exellent” 8 scores

“good” 6 scores

“satisfactory ” 5 scores

Module 3

80

20 tests “Krok 1” 20 scores

4 questions:

“exellent” 9 scores

“good” 7 scores

“satisfactory ” 6 scores


3 questions

“exellent” 8 scores

“good” 6 scores

“satisfactory ” 5 scores

Learning Outcomes Evaluation Table for Conversion

from national 4-point scale to ECTS Systems

ECTS scale

National scale

BSMU scale

^ A (Excellent)

excellent

180-200

B (Very Good)

good

165-179

C (Good)

150-164

D (Satisfactory)

satisfactory

135-149

E (Sufficient)

120-134

FX (Fail)

unsatisfactory, recurrence of the course material is necessary in order to receive a passing credit

70-119

F (Fail)

unsatisfactory, recurrence of the entire course is necessary

1-69

Assesment criteria. Descriptions of what the learner is expected to do, in order to demonstrate that a learning outcome has been achieved.

Excellentexcellent performance with a negligible amount of mistakes.

Very goodabove the average standard but with some errors.

Goodgenerally correct work with a definite amount of significant mistakes.

Satisfactoryfair but with significant shortcomings.

Sufficientperformance meets the minimum criteria quality of knowledge.

Those who have not achieved a performance sufficient to allow a passing grade are divided into two subgroups:

FX – Fail – some more work required before credit can be awarded;

F – Fail – considerable further work is required. This distinction allows differentiation between those students who have been assessed as almost passing and those who have clearly lacked the required knowledge and skills.


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