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ACTION OF EPEC AND ANTIBIOTICS ON MICROFLORA OF INTESTINE OF CHILDREN WITH INTESTINAL ESCHERICHIOSIS
Аhmed Heblo, 3-th year student
Scientific supervisor – candidate of medical science V.V. Lipovskaya
Sumy State University, chair of infectious diseases and epidemiology with course of microbiology, virology, immunology
Change of qualitative and quantitative structure contents of the microflora of intestines under influence Enteropathogenic E. coli (ЕРЕС) and antibiotic therapy on 98 children Sumy in the age 2 months till 8 years is investigated. The contents of microflora in excrements was investigated according to methodical recommendations “Laboratory diagnostics of dysbacterioses”.
Such statistically authentic changes autochthonous microflora of intestines at children. Have been revealed: Bifidobacterium spp. at all surveyed children their amount changed within the limits of 105-107 CFO/1g feces, that considerably below norm. 55.1% of children the contents Bifidobacteria has made 106 CFO/1g feces and only 22.4% of patients their amount reached some 107 CFO/1g feces. Decrease in the contents of Lactobacteria up to a parameter of 103-106 CFO/1g feces is revealed also. 46.9% of children the amount Lactobacteria has made 103 CFO/1g feces and 34.7% of children their contents reached some 105 CFO/1g feces. The contents Bacteriodes at all patients has been lowered, did not exceed a parameter of 106 CFO/1g feces. Significant changes have been revealed in qualitative and quantitative structure of contents E. coli. At 72.2% of patients dysbiotic changes were characterized by total high-grade decrease in fermentation attitude of E. coli. Its contents was less than 106 CFO/1g feces. 28.6% of patients are revealed lactose negative serotypes Escherichia and 41.9% of patients – serotype E. coli with lowered fermentation activity. The quantity of lactose negative E. coli has made 107 CFO/1g feces, and the contents E. coli with lowered fermentation activity made 105 CFO/1g feces. Of all serotypes of E. coli 68% serotype showed lytic and hemolytic effect on donor specific bacteriophage MS2. It is found out, that Bifidobacteria and Lactobacteria deficiency was connected with 100 % hemolytic activity of E. coli. The contents of conditional-pathogenic bacteria has been raised and also bacteria formed associations.
Analyzing the received results, it is possible to approve powerful influences of ЕРЕС and antibiotic therapy on qualitative and the quantitative structural contents of children intestinal microflora.
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