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Spectrum Analysis of Absorbing Lung Condensat
Spectrum Analysis of Some Organic Substances of Lung Condensat.
Absorbent and dispersive pecularities of man’s lung condensant and its parts.
S.G. Guminetsky1, A.V. Motrich1, Chernivtsy National University named after Yu. Fedjkovych 1, M.M. Kuzjmin2, Bukovina State Medical Academy 2.
Abstract: Given spectrum of absorbent lung condensat of healthy people (controlled people) tubercular patients , and organic parts in a spectral interval λ = 220 – 340 nm, measured(using Buger and Ber’s law) and with the help of spherical photometer.
Their equality gives us the opportunity to determine the presence of dispersion in lung condensat, and on some of its parts. Here we give the opportunity of using received results in a clinical practice for diagnostics tuberculosis of lungs.
Keywords: spectrum of absorbing, lung condensat, biological-active substances.
In the medical research practice, being described in a special scientific literature [1-4], we come across more often the works about the using humidity of air, that is breathed out by the lungs of a man (lung condensat),for determing bronchi system desease, preasthma, heart defect and other unspecifical chronical ailments.But there is practically no information about lung condensat researches of tubercular patients in the literature, especially using optical methods.Conserning this, the purpose of the work is: researching of absorbing spectrum of lung condensat examples of healthy people and tubercular patients in a spectral interval λ = 220-340 nm, and also biological-active substances (BAS), which can be present in it, according to the literature statistics: free amino acids (triptophan and phenilanin) and cortisone, lecetin and holing.
The examples of lung condensat has been prepared at a phtisiatry department of the Bucovina State Medical Academy with the help of typical system: the patient is breathing about 15 minutes in a device for collecting of condensate - spiral glass tube, cooled to 0 ˚C. During this time the size of isolated condensat was: for healthy patients 4-5 ml, and for sick 2-3 ml, so for the sick tubercular patients the isolation activity of lung condensat is, as a rule, less, than for healthy. There were tested 10 sick people and 10 healthy aged 20-30 years old.
For example, at the draw. 1 there are typical absorbing spectrum D = f(λ) of lung condensat for one of the healthy patients, and at the draw. 2- for the sick tubercular patient. Here curves 1 were received on a spectrum photometer SF-46 using Buger and Ber’s law, and curves 2 were received using spherical photometer method , which give the opportunity at the condition of only dispersion to get the original absorbing spectrum without dispersion influence. We can see that in the area λ < 320 nm, in which there are electronic absorbing spectrum of organic parts of biological-active substances, including free amino acids, in the lung condensat of tubercular patients is much lower, than of healthy person. It can occur practically in all cases from ten lung
condensat, optical omissions D of lung condensat of sick people are much lower than of healthy people. According to the literature statistics [2,3] we can suppose, that the summary of studied sick and ten healthy patients. It also shows the presence of maximum in absorption in the area λ = 285 nm for the condensat of healthy patients, conditioned by the presence of triptophan in it, at the same time in the condensat of sick patients it is not cleared up. Besides, comparing drawings 1 and 2, their curves, follows that for condensat of healthy patients optical omissions in all researched spectral interval, received with the help of spherical photometer, are much lower from that, that were received on the spectrophotometer SF-46, that were not observed in the case tubercular patient condensat, for what the curve 1 and 2 are practically coincided. The result of it is that in the mixture of tubercular patient condensat there are no organic substances, which result to the dispersion.
To clear up what parts and how they determine the dispersive peculiarities of lung condensat of a person, we have researched the absorbent and dispersive peculiarities for some of their parts, first of all hormones. We have made their solutions of different concentration and researched spectrum of absorbing in the interval λ = 220-460 nm by two methods spoken above. The results of the research with the lecetin are given at the drawing 3, where the curve 1 is received on SF-46, and the curve 2 is got with the help of spherical photometer: the concentration of lecetin in the solution C = 180 mg%. We can see, that the solution of lecetin is optically unhomogeneous medium, the result of which its optical omissions D in the all studied spectral interval is much higher from their true meanings. The similar situation was also with solutions of cortisone and holing, but for cortisone the difference between curves 1 and 2 is much bigger, and for holing is much lower and is seen when λ>320 nm. It means that dispersive pecularities of BAS differ much.. Besides, it was admitted, that the solutions of amino acids, triptophan and phenilanin are optically homogeneous. For comparison between one another absorbent and dispersive peculiarities of researched organic elements of lung condensat at the draw. 4a and 4b are given spectral dependences of their optic omission for the solutions of the same concentration C = 90 ml%, received on SF-46. Here the curve 1 is related to the holing, the curve 2- for lecetin and 3- for cortisone. It is showed. that the biggest influence upon the absorbing spectrum of lung condensat can result to the presence in it of cortisone and minimum of holing.
Presence of Amino Acids in the Lung Condensat.
Using analytical method of absorbing spectral analysis we can value quantitative content of phenilanin and triptophan in the lung condensat. For this purpose we use spectral curves for indicated amino acids, which are given in the work (6), taking in the quality of analytical length of the wave for phenilanin λ = 265nm, and for triptophan λ = 285 nm. Then the formulas for calculations of concentration Cf - phenilanin and Ctf -triptophan are:
Here 1-is a width of the tube is equal to 1 sm , , , division of omission for phenilanin and triptophan for given lengthes of waves.
The results of calculations for some patients are given in tab.1
Table 1. Value of concentrations (in mg%) aromatic amino acids in the lung condensat.
Table 2. Value of concentrations Cf and Ctf, in mg%.
Table 3. Value of relative errors Δ of concentration determination Cf and Ctf, in %.
We can see, that the concentration of free amino acids in these cases in the lung condensat of sick tubercular patients is lower, than of healthy people, and there are always more phenilanin than triptophan. For making evaluation of exactness of this method for determination of phenilanin and triptophan in a two-component solution, we have made series of solutions with given concentration values of phenilanin and triptophan: for phenilanin C = 0,4; 0.8; 1,2; 1,6; 2,4 and 3,2 mg%; for triptophan C = 0,2; 0,4; 0,8; 1,6 mg%, so, in the real borders of presence these amino acids in the lung condensat of the man (tab. 1). There were 24 solutions altogether. We have researched absorbing spectrum in the interval λ = 240-320 nm. Choice of the spectral area is determined by the location in the spectrum of analytical stripes of absorption for phenilanin and triptophan . Measuring were held on spectrophotometer SF-46 using Buger-Ber’s law.
The results of calculations according to the formulas (1) and (2) of the phenilanin concentration Cf and triptophan Ctf for each from 24 solutions given in the tab. 2.
Comparing calculated values with given, we can evaluate the exactness of the method of concentration determing of phenilanin and triptophan in the two-component solution in dependence from combination of size Cf and Ctf. Calculated relative errors are given in tab. 3.
We can make the conclusion that minimal error in determination of concentration phenilanin and triptophan in two-component solution is seen for average values of concentrations: 0,8-1,6 mg % for phenilanin and 0,4- 0,8 mg% for triptophan, and also at the time when concentrations of both components on the solutio are close between each other.
The received in this work results give the opportunity to set proper quantitative criterions , that characterize the differences between the properties of the lung condensat of the healthy people and sick tubercular patients:
Given criterion can be used in routine laboratory practice of antitubercular establishments as one of the ways of diagnostics of lung tuberculosis.
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