Thematic plan of practical lessons on microbiology, virology and immunology icon

Thematic plan of practical lessons on microbiology, virology and immunology




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Thematic plan of practical lessons on microbiology, virology and immunology

for students of 2 year of pharmaceutical faculty (speciality- Clinical Pharmacia)



N

less

Number of hours

Theme of practical classes


Credit N1. Morphology, physiology of microorganisms. Infection. Immunity.

Thematic credit 1. Introduction in microbiology.

1

2

Working regime of microbiological laboratory. The rules of the work in the bacteriological laboratory. Microscopy techniques. Immersion microscopy of bacterial smears.

^ Thematic credit 2. Morphology and structure of the prokaryotes and unicellular parasitic eucaryotes.

2

2

Morphology of bacteria. Preparation of the smear from bacterial culture. The simple method of staining.

3

2

Structure and chemical composition of bacterial cell. The structure of the cell wall. L-forms bacteria. The complete methods of staining. Gram’s method.

4

2

Acid-fast bacteria. Bacterial endospores. Methods of their staining with Aujesko`s and Zielh-Neelsen`s techniques.

5

2

Capsule of bacteria. Intracellular inclusions. Methods of their revealing.

6

2

Flagella. Methods of their revealing.

7

2

Morphology of spirochetes, mycoplasma and actinomycetes. Features of their structure and methods of staining

8

2

Morphology of rickettsia, Chlamydia. Features of their structure and methods of staining

9

2

Morphology of microscopic fungi. Features of their structure and methods of staining

10

2

Morphology of protozoa. Features of their structure and methods of staining

^ Thematic credit 3. Physiology of the microorganisms. Evolution and classification of the germs.

11

2

Physiology of the microorganisms. Nutrition of bacteria. Isolation of a bacterial pure culture (first stage).

12

2

Physiology of the microorganisms. Multiplication of bacteria. Isolation of a bacterial pure culture (second stage).

13

2

Physiology of the microorganisms. Bacterial enzymes. Biochemical activity of bacteria. Isolation of a bacterial pure culture (third stage).

14

2

Physiology of the microorganisms. Bacterial respiration. Cultivation of anaeaerobs. Pure culture isolation of an anaerobic micrororganism.

^ Thematic credit 4. Bacterial genetics.

15

2

Bacterial genetics. Variation of bacteria. Brief review of the genetic engineering. Transformation. Transduction. Conjugation.

^ Thematic credit 5. Microbiological base of an antimicrobial chemotherapy and asepsis.

16

2

Influence of the physical and chemical factors on microorganisms. Sterilization techniques..

17

2

Chemotherapeutical drugs. Antibiotics. Determination of a bacterial susceptibility to antimicrobial drugs.

^ Thematic credit 6. Infection.

18

2

Infection. Factors of a bacterial pathogenicity, their detection. Biological method of a laboratory diagnostics.

Thematic credit 7. Immune system. Non-specific defense from pathogens.

19

2

Species immunity. Detection of the nonspecific resistance factors.

^ Thematic credit 8. Antigens and antibody. Immunity reactions.

20

2

Acquired (specific) immunity. Antigens and antibodies. Serological method of the laboratory diagnostics of infectious diseases. Agglutination test, its modifications.

21

2

Serological tests. Precipitation tests, its modifications.

22

2

Serological tests. Complement fixation test.

23

2

Serological tests with labelled antibody or antigen, their application in the laboratory diagnostics.

^ Thematic credit 9. Immunopathology. Immunoprophylaxis and immunotherapy of infectious diseases.

24

2

Immunity. Allergens. Manufacture of microbial allergens. Microbial allergy, methods of investigation and diagnostics.

25

2

Immunoprophylaxis and immunotherapy of infectious diseases. Vaccines and toxoides.

26

2

Immunoprophylaxis and immunotherapy of infectious diseases. Immune serums and immunoglobulin.

27-28

4

Practical skills to module N1

29

2

Summary control of the credit N1. Morphology, physiology of microorganisms. Infection. Immunity.

Credit N2. Special, clinical and ecological microbiology.

Thematic credit 10. Pathogenic prokaryotes and eucaryotes

30

2

Rickettsia. Biological properties. Laboratory diagnostics of rickettsiosis. Serological diagnostics of the epidemic and endemic (murine) typhus, Q-fever.

31

2

Chlamydia. Biological properties. Laboratory diagnostics of chlamydiosis.

32

2

Pathogenic cocci. Staphylococci. Morphology and biological properties. Laboratory diagnostics of suppurative and non-suppurative lesions caused by them.

33

2

Pathogenic cocci. Streptococci. Morphology and biological properties. Laboratory diagnostics of diseases.

34

2

Pathogenic Gram-negative cocci. Gonococci and meningococci. Biological characteristics and laboratory diagnostics of diseases.

35

2

Enterobacteria. Biological characteristics and classification of family. Characteristics of genera Escherichia. Pathogenesis, laboratory diagnostics of coli-enteritis.



A chief of microbiology, virology and immunology department

Academician AS HS of Ukraine

Doctor of medicine, professor G.K.Paliy

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